Welcome to Whonix forums and thank you for your question!
Unfortunately this is undocumented, unsupported at time of writing.
Undocumented, Untested or Unsupported Features
Doable in theory. Known to work in Qubes-Whonix.
How-to: Ledger Hardware Wallet in Kicksecure ™
Whonix does not take deliberate steps to prevent use of Ledger. But:
- Using Ledger on (Debian) Linux can be challenging. (non-Whonix issue)
- Using USB devices with VirtualBox can be challenging. (non-Whonix issue)
- Using Ledger with VirtualBox can be challenging. (non-Whonix issue)
Once you’ve mastered above issues (as per Free Support for Whonix ™), Whonix will probably not in your way.